Fig. 3: Significant increases in multiple substrates and products of the one-carbon metabolism in all host tissues indicate this pathway may be involved in cachexia aetiology.
a, A graphical overview of one-carbon metabolism and related metabolic pathways. THF, tetrahydrofolate; DMG, dimethylglycine; GSH, reduced glutathione; GSSG, oxidised glutathione; SAH, S-adenosyl homocysteine; MTR, 5-methylthioribose; MTA, 5′-methylthioadenosine. b, A table showing substrates and products related to one-carbon metabolism, assigned to a specific identification number (ID). c–g, Bar graphs showing relative levels of substrates and products of one-carbon metabolism in Non-cax, Pre-cax and Cax tumour mice versus healthy controls (Ctrl), in plasma (c), liver (d), eWAT (e), heart (f) and GC muscle (g). n = 4 animals per group. See also Fig. 1a for the experimental set-up, and Extended Data Fig. 4. Data are the mean ± s.e.m. Statistical analysis based on raw data (MS signal intensities, arbitrary units (AU)). One-way ANOVA with Dunnett’s post-hoc tests versus Ctrl or Kruskal–Wallis with Dunn’s post-hoc tests versus Ctrl. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with the Ctrl group. N.D, not detected.
