Extended Data Fig. 3: ScURA restores cell proliferation in SDHA knockout cells upon mETC inhibition. | Nature Metabolism

Extended Data Fig. 3: ScURA restores cell proliferation in SDHA knockout cells upon mETC inhibition.

From: Ectopic expression of cytosolic DHODH uncouples de novo pyrimidine biosynthesis from mitochondrial electron transport

Extended Data Fig. 3: ScURA restores cell proliferation in SDHA knockout cells upon mETC inhibition.

a, Left, immunoblot showing SDHA knock out in 143B WT cells and re-expression of ScURA. Right, average number of doublings per day of 143B SDHAKO cells treated with antimycin/myxothiazol or vehicle, with or without exogenous uridine supplementation. b, Left, immunoblot showing SDHA knock out in U2OS cells and re-expression of ScURA. Right, average number of doublings per day of U2OS SDHAKO cells treated with antimycin/myxothiazol or vehicle, with or without exogenous uridine supplementation. Data are mean ± SD, n = 3 independent biological replicates. Ordinary one-way ANOVA, Sidak’s multiple comparison test, ****P < 0.0001, **P < 0.01, *P < 0.05.

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