Fig. 1: The circadian clock regulates respiration at the mitochondrial respiratory chain CI.
From: Adipocyte NADH dehydrogenase reverses circadian and diet-induced metabolic syndrome
a, OCR in gWAT mitochondria isolated from control (Bmal1loxP/loxP) and Bmal1 KO (Adipoq-cre; Bmal1loxP/loxP) mice at ZT2 and ZT14 in the presence of the indicated substrates and inhibitors (n = 7 control and n = 6 Bmal1 KO for pyruvate (Pyr), and n = 6–7 control and n = 5 for Bmal1 KO for succinate (Succ)). b, State III respiration in gWAT mitochondria isolated from control and Bmal1 KO mice at ZT14 in response to CI-linked and CII-linked substrates (n = 5). c, OCR in gWAT mitochondria of control and Bmal1 KO mice at ZT2 and ZT14 in the presence of the indicated substrates and inhibitors (n = 5). d, OCR in gWAT mitochondria of wild-type mice at ZT2 and ZT14 after 12 h of fasting in response to the indicated substances as in c (n = 5). e, State III respiration in adipocytes differentiated from control and Bmal1 KO mice in the presence of the CI–IV substrates (n = 5). f, NADH-dependent OCR in adipocytes differentiated from control and Bmal1 KO mice (n = 6). g, NADH-dependent OCR in control, Bmal1−/− and Cry1/2−/− MEFs (n = 6). Data are presented as the mean ± s.e.m. a,c, Statistical significance was calculated using a two-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test with the ZT14 control set as the reference group. b,e, A multiple unpaired two-sided t-test was used. d, A two-way ANOVA followed by multiple comparisons test was used. f, An unpaired two-sided t-test was used. g, A one-way ANOVA followed by multiple comparisons test was used. The # symbol denotes the statistical significance between ZT14 control and all other groups. *P < 0.05, **P < 0.01, ***P < 0.001.
