Extended Data Fig. 7: Loss of Ndufs2 in adipocytes leads to reduced complex I and III activity in gWAT, related to Fig. 3.
From: Adipocyte NADH dehydrogenase reverses circadian and diet-induced metabolic syndrome
a, SDS-PAGE immunoblot of Ndufs2 in isolated mitochondria from Control (Ndufs2fx/fx) and Ndufs2-KO (Adiponectin-Cre; Ndufs2fx/fx) mice at 3 months of age on a chow diet (n = 2). b, SDS-PAGE immunoblot of electron transport chain proteins in isolated gWAT mitochondria from Control and Ndufs2-KO mice at 3 months of age (n = 3-4). c, Quantification of protein abundance of samples in B. d, OCR in gWAT and iWAT mitochondria of Control and Ndufs2-KO mice at ZT14 in the presence of indicated substrates and inhibitors (n = 7 for gWAT and n = 6 for iWAT). TMPD, Tetramethyl-p-phenylenediamine; Asc acid; ascorbic acid. e, OCR of differentiated adipocytes from Control and Ndufs2-KO mice in the presence of permeabilizer (PERM), ADP, and complex I to IV substrates followed sequentially by oligomycin and antimycin A (n = 5). Data are represented as mean ± SEM. Statistical significance was calculated by two-way ANOVA followed by multiple comparisons in c, d, and e.*p < 0.05, **p < 0.01, ***p < 0.001.
