Extended Data Fig. 1: The circadian clock regulates mitochondrial complex I respiration, related to Fig. 1.
From: Adipocyte NADH dehydrogenase reverses circadian and diet-induced metabolic syndrome
a, OCR in iWAT mitochondria of Control and Bmal1-KO mice at ZT2 and ZT14 in the presence of indicated substrates and inhibitors (n = 4). TMPD, Tetramethyl-p-phenylenediamine; Asc acid; ascorbic acid. b, Representative bioluminescence of PER2::LUC oscillations in synchronized primary white adipocytes from mPer2:Luc mice. c, Relative mRNA expression of Bmal1 in synchronized white adipocytes in vitro (n = 6 per timepoint). d, NADH-dependent respiration in synchronized white adipocytes in vitro (n = 6 per timepoint). Data are represented as mean ± SEM. Statistical significance was calculated by two-way ANOVA followed by Dunnett’s multiple comparisons test with ZT14 Control set as the reference group in a.
