Extended Data Fig. 3: Circadian control of SAM-dependent NDUFS2 methylation, related to Fig. 1.
From: Adipocyte NADH dehydrogenase reverses circadian and diet-induced metabolic syndrome
a, Model illustrating how BMAL1 regulates methionine metabolism and SAM production through Mat2a, enabling NDUFAF7-mediated methylation of NDUFS2 required for proper complex I assembly. b, SAM levels in Control, Bmal1−/− and Cry1/2−/− MEFs (n = 5 technical replicates per genotype). c, Methylarginine and NDUFS2 western blotting of immunoprecipitated NDUFS2 in Control, Bmal1−/− and Cry1/2−/− MEFs (n = 2). Data are represented as mean ± SEM. Statistical significance was calculated by one-way ANOVA followed by multiple comparisons in b. * p < 0.05, **p < 0.01, ***p < 0.001.
