Extended Data Fig. 4: High fat diet feeding leads to reduced and loss of diurnal CI respiration in visceral WAT mitochondria, related to Fig. 2.
From: Adipocyte NADH dehydrogenase reverses circadian and diet-induced metabolic syndrome
a, OCR in WAT mitochondria of wildtype mice at ZT14 following 12 weeks of chow or HFD feeding. Mitochondria were treated with pyruvate/malate/FCCP followed sequentially with rotenone, succinate, antimycin A, and N,N,N′,N′-Tetramethyl-p-phenylenediamine (TMPD)/ascorbic acid (n = 5). b, OCR in WAT mitochondria of wildtype mice at ZT2 and ZT14 following 12 weeks of HFD feeding. Mitochondria were treated with pyruvate/malate/NADH followed sequentially with rotenone/antimycin A, TMPD/ascorbic acid, and azide (n = 6). Data are represented as mean ± SEM. Statistical significance was calculated by two-way ANOVA followed by multiple comparisons in a-b. **p < 0.01.
