Extended Data Fig. 6: Impact of altering absolute redox levels by oxidant or pro-oxidant on physical function in aged mice.
From: Redox rhythms promote fitness by modulating ageing-dependent reprogramming
a-b, Blood glucose levels of ITT (a, YW n = 8, OW n = 9, ON n = 6, OM n = 3) and GTT (b, YW/OM n = 6, OW/ON n = 5) were measured across time after injection at active phase, and with corresponding AUC (right) shown. c-d, Serum insulin concentrations (c, n = 5 per group) and fasting blood glucose concentrations (d, YW n = 8, OW n = 9, ON n = 6, OM n = 3) in individual groups after fasting for 4 h. e-i, Bar plots showing the assessment of overall physical and locomotion functions, including grip strength (e, YW n = 14, OW n = 8, ON n = 6, OM n = 5), running distance on the treadmill (f, YW n = 9, OW n = 14, ON n = 6, OM n = 4), latency to fall (g, YW n = 13, OW n = 15, ON/OM n = 4), forelimb and hindlimb stride lengths (h-i, YW n = 17, OW n = 7, ON n = 6, OM n = 3) in individual groups. Data are presented as means ± SEM. P values were calculated using (a-right panel, b-right panel, c-i) one-way or (a-left panel, b-left panel) two-way analysis of variance (ANOVA) with Bonferroni post-hoc corrections. Only significant comparisons are labelled (*, YW vs. OW; * P < 0.05, **** P < 0.0001).
