Extended Data Fig. 8: OP1-ND intervention significantly reversed the ageing-related features in liver and skeletal muscle.
From: Redox rhythms promote fitness by modulating ageing-dependent reprogramming
a, Relative H2O2 levels at ZT18 in LV (left) and SM (right) of aged mice treated with DS from ZT10.5 to ZT18 for one week, compared to water controls (n = 4 per group). b, Body weight of mice from different groups (YW n = 15, OW/OP1-ND n = 20, OD n = 12). c, Blood glucose levels in GTT were measured at multiple time points after intraperitoneal injection of glucose (left), and corresponding AUC were shown (right) (YW n = 10, OW n = 13, OP1-ND/OD n = 12). d, The ratio of skeletal muscle fibres of different sizes to the total number of fibres (YW n = 5, OW/OP1-ND n = 9, OD n = 8). e, Proportional distribution of the mitochondrial area in individual groups, assessed by transmission electron microscopy (YW n = 3, OW/OP1-ND/OD n = 4). f, Relative mitochondrial DNA copy number, determined as the ratio of mitochondrial genes (D-loop and Cytb) to nuclear DNA (18S) in SM of individual groups (YW n = 7, OW/OP1-ND n = 9, OD n = 5). g, IL-6 concentrations in plasma were measured by ELISA (YW/OP1-ND/OD n = 7, OW n = 8). Data are presented as means ± SEM. P values were calculated using (a) two-tailed unpaired t-test, (b, f-Cytb, g) Kruskal–Wallis followed by Dunn’s multiple comparisons hoc test or (c-right panel, f-D-loop) one-way or (c-left panel, d-e) two-way analysis of variance (ANOVA) with Bonferroni post-hoc corrections. Only significant comparisons are labelled (*, YW vs. OW; #, OP1-ND vs. OW; * or # P < 0.05, ## P < 0.005, *** or ### P < 0.001, **** or #### P < 0.0001).
