Extended Data Fig. 4: Multiple glycans exhibit increased biosynthesis but unchanged turnover in 5xFAD mouse brains.
From: Hyperglycosylation is a metabolic driver of Alzheimer’s disease
a, Spatial distribution and quantification of 13C-labeled isotopologues of glycan 1688 m/z following isotope pulse in WT and 5xFAD mice. Bar graphs show significantly increased 13C incorporation across isotopologues in 5xFAD. Washout after 24 hours reveals no significant difference in turnover between genotypes (n = 3 animals per group). Data are mean ± s.e.m. P values are indicated (two-way repeated-measures ANOVA). b, Enrichment and washout analysis of glycan 1282 m/z demonstrates elevated biosynthesis in 5xFAD with no change in degradation rates (n = 3 animals per group). Data are mean ± s.e.m. P values are indicated (two-way repeated-measures ANOVA). c, Similar enrichment profile observed for glycan 1444 m/z, showing significantly increased labeling in 5xFAD without altered clearance (n = 3 animals per group). Data are mean ± s.e.m. P values are indicated (two-way repeated-measures ANOVA).
