Extended Data Fig. 5: Hyaluronan and O-GlcNAcylation are reduced in 5xFAD and PS19 mouse brains. | Nature Metabolism

Extended Data Fig. 5: Hyaluronan and O-GlcNAcylation are reduced in 5xFAD and PS19 mouse brains.

From: Hyperglycosylation is a metabolic driver of Alzheimer’s disease

Extended Data Fig. 5: Hyaluronan and O-GlcNAcylation are reduced in 5xFAD and PS19 mouse brains.The alternative text for this image may have been generated using AI.

a, Schematic of the hexosamine pathway showing production of N-acetyl-glucosamine for N-glycans, hyaluronan and protein O-GlcNAcylation. b, Workflow for on-tissue hyaluronan imaging: brain sectioning, antigen retrieval, hyaluronidase treatment, CHCA matrix application and MALDI–MS analysis. c, Hyaluronan imaging and violin plots of absolute intensities showing decreased brain hyaluronan levels in 5xFAD and PS19 mice compared with WT controls. d, Immunofluorescence staining of O-GlcNAcylation in WT versus 5xFAD (top) and WT versus PS19 (bottom) brains, with quantification demonstrating reduced O-GlcNAcylation signal in both disease models (n = 9 animals per group). Data are mean ± s.e.m.; P values are indicated (two-tailed t-test). Panels created in BioRender: a, Sun, R. https://biorender.com/aht5d7z (2026); b, Sun, R. https://biorender.com/k69h363 (2026).

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