Extended Data Fig. 3: Sequencing analyses of immortalized and anchorage-independent chicken fibroblasts. | Nature Food

Extended Data Fig. 3: Sequencing analyses of immortalized and anchorage-independent chicken fibroblasts.

From: Spontaneous immortalization of chicken fibroblasts generates stable, high-yield cell lines for serum-free production of cultured meat

Extended Data Fig. 3: Sequencing analyses of immortalized and anchorage-independent chicken fibroblasts.

(A) K-mean clustering using Over-Representation (OR) Analysis of CEF-2 and HUN-CF-2. Full list of pathways (1-24) is provided in Supplementary Table 1 (S1A). Green: Downregulated genes, red: Upregulated genes. (B) K-mean clustering using Gene Set Enrichment (GSE) Analysis of CEF-2 and HUN-CF-2. Full list of pathways (1-20) is provided in Supplementary Table 1 (S1B). Green: Downregulated genes, red: Upregulated genes. (C) A heat map based on copy number variations (CNVs) of four immortalized chicken fibroblast lines, with FMT-SCF-1 and −2 derived from broiler Ross 308 and FMT-SCF-3 and −4 derived from Israeli Baladi chicken, respectively. Green: normal copy of genomic sequences (2n), purple: deletions of one copy (1n), turquoise: 2-copy deletion (0n). Red: (3n), orange: (4n) and black: (> 5 copies) mark duplication events. Boundaries between chromosomes are labelled black due to the repetitive sequence of the telomeres. (D) A phylogenetic tree of the four immortal chicken fibroblast lines based on similarities and differences in CNVs. (E) PCA of primary cells from broiler Ross 308 (CEF-2), Israeli Baladi (CEF-4), immortalized adherent and immortalized suspension lines of East Lansing (DF-1). Scale bar equals 100 μm.

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