Extended Data Fig. 3: Development of a perfused chicken fibroblast culture with contiguous partial harvests. | Nature Food

Extended Data Fig. 3: Development of a perfused chicken fibroblast culture with contiguous partial harvests.

From: Empirical economic analysis shows cost-effective continuous manufacturing of cultivated chicken using animal-free medium

Extended Data Fig. 3

(a) Multiple TFF experiments showing first ten days following inoculation in SFM and ACF, respectively. TFF circulation indicates the day on which the bioreactor suspension started circulating in the TFF loop. (b) TFF circulation rate is identical to the low-shear magnetic levitation pump output, describing the circulation speed in the TFF loop. (c) Shear rate as a result of TFF circulation rate does not exceed 2500 s−1 to prevent shear damage. (d) Contiguous harvest experiments in SFM. (e) Metabolite concentrations in contiguous harvest experiments in SFM. (f) Contiguous harvest experiments in ACF. (g) Metabolite concentrations in contiguous harvest experiments in ACF. (h) Summary of run parameters for contiguous harvest experiments in SFM and ACF. Average plus/minus standard error of the mean of two experiments in SFM, and three experiments in ACF, respectively. (i) Filtrate flux and Cell-specific perfusion rate (CSPR) in SFM and ACF experiments in lab-scale.

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