Extended Data Fig. 10: Olaparib-treated macrophages suppress T-cell function, which is overcome with anti-CSF-1R therapy in BRCA-deficient TNBC.
a, BT20 or MCF7 human breast tumor cells were treated with conditioned media from IL-4 plus GM-CSF differentiated myeloid cells in the presence or absence of Olaparib. b, Control media was generated similar to conditioned media but was not incubated with monocytes; it did not induce tumor cell killing. Error bars represent standard error of mean (±SEM). Statistical analyses were performed using one-tailed t-test. Only one replicate for data in B. c,d, OT-1 T cells cultured in supernatants collected from media with vehicle (red), media with Olaparib (blue), human macrophages treated with vehicle (black, donors 1-3), or human macrophages treated with Olaparib (light blue, donors 1-3) were assessed for (c) live cell number and (d) AnnexinV (n = 3 human donors). Error bars represent standard error of mean (±SEM). Statistical analyses were performed using paired t-test or one-way ANOVA as indicated on graphs. e-g, CD8 T-cells are effectively depleted with anti-CD8 antibodies, corresponding to Fig. 6d (n = 5 mice/group). Frequency of CD8+T-cells in tumors (e) and (f) are shown. Gating strategy to gate CD8+T-cells is shown (g). Error bars represent standard error of mean (±SEM). Statistical analyses were performed using one-tailed t-test. h, Flow plots corresponding to Fig. 6h-j. Exact p values indicated in each panel for each comparison.
