Extended Data Fig. 10: CAR-NKT cells promote T cell responses to neoantigens in the BBN996 bladder tumor model.
(a) Representative flow cytometry plots showing the expression of hCD19 on BBN963 cell line. Pink histogram illustrates control cells. n = 4 independent experiments with similar results. (b, c) Representative flow cytometry plots (b) and summary (c) showing coculture of CAR-NKT or CAR-T with BBN963-hCD19 tumor cells. T/NKT cells were cocultured with tumor cells at an E:T ratio of 1:1 for 5 days. On day 5, all cells were collected and analyzed by flow cytometry to quantify tumor cells (hCD19+), n = 3 mice; ordinary one-way ANOVA. (d) IFN-γ was detected by ELISA in the coculture supernatant of CAR-NKT or CAR-T cells with BBN963-hCD19 after 24 hrs. Data are shown as the mean ± SD; n = 3 mice; ordinary one-way ANOVA. (e) Schematic of the BBN963-hCD19 bladder tumor model. (f, g) Representative image of tumor (f), measurement of tumor volume (g) after tumor engraftment and CAR-T or CAR-NKT administration in the model described in (e). Data are shown as the mean ± SD; n = 5 mice per group; two-way ANOVA. (h) Survival curve of the BBN963-hCD19 model described in (e); n = 5 mice per group; Kaplan-Mayer analysis with log-rank test. (i, j) Summary of BBN963 neoantigen specific T cells (h) and NK cells (i) in the tumor model described in (e); ordinary one-way ANOVA.
