Extended Data Fig. 3: Metastatic tumors in RPM model establish with high penetrance NEPC histology and marker profile.
From: The neuroendocrine transition in prostate cancer is dynamic and dependent on ASCL1
a. Representative ASCL1 IHC stains from iliac lymph node metastases isolated from RPM OT mice. Dotted line represents the boundary of tumor and normal tissue. b. Representative serially sectioned immunohistochemical stains from metastases isolated across (top) liver and (bottom) lung tissue in RPM OT mice. Scale bars denoted in figure legend. VIM = VIMENTIN, SYP = SYNAPTOPHYSIN. c. Representative H&E stains and histological grade of metastases isolated from regional lymph nodes, kidney, liver, and lung tissue from RPM OT mice. Data related to panels a-b. Scale bars denoted within the figure panel. d. Pie charts demonstrating percentage of mice harboring distinct histotypes of prostate cancer in the indicated metastatic regions. Sample size denoted in figure panel. e. Representative mIF staining for lineage markers in metastastic tumors isolated from regional lymph nodes, liver, and lung tissue from RPM OT mice. Data representative of n = 3 mice. f. Percentage of unique cell types expressing the indicated lineage markers in RPM metastatic samples. For panels a-d, images and data are derived from n = 25 lymph node, n = 5 liver, and n = 9 lung metastatic samples from RPM OT mice. For panel e-f, images and data are representative of n = 3 lymph node, n = 3 liver, and n = 3 lung metastatic samples from RPM OT mice. Error bars denote mean and s.d. All scale bars and pseudo-coloring denoted in the figure panel.
