Extended Data Fig. 1: Engineering of clinically relevant mutations within an allelic mouse prostate organoid series.
From: The neuroendocrine transition in prostate cancer is dynamic and dependent on ASCL1
a. Oncoprint of the indicated genes frequently mutated in human primary prostate cancer. Data (n = 1633 patient samples) obtained from the studies indicated within the figure legend. b. Schematic of the lentiviral vector used within this study to overexpress cMycT58A transcriptionally linked to EGFP in organoids. c. Representative brightfield (left), GFP fluorescence (center), and hematoxylin & eosin (H&E) stains (right) of organoids harboring mutations in indicated tumor suppressors and oncogenes. Images are representative of n = 3 technical replicate organoid samples per genotype. H&E and GFP imaging were repeated independently twice with similar results. d. Representative confocal images of 7-plex mIF stains of organoids of the indicated proteins. Data are representative of n = 3 technical replicates per genotype. e. Percentage of unique cell types expressing the indicated markers in organoid culture. Data representative of n = 3 technical replicates and related to images in panel d. Error bars denote mean and s.d. All scale bars and pseudocolor legends indicated within the figure panel.
