Extended Data Fig. 10: TP53/RB1 knockout in human normal airway organoids.
(a) The growth of human normal lung airway organoids (n = 1 line) (left) and TR-KO lung airway organoids (n = 1 line) (right) cultured with the indicated conditions. Scale bar: 500 mm. Three independent experiments were evaluated with similar results. (b) Representative ASCL1, NEUROD1, POU2F3 and YAP1 staining in the TR-KO lung airway organoids (n = 1 line). Scale bar, 100 mm. Three independent organoids were evaluated with similar results. (c) Representative H&E, SYP, CHGA and CD56 staining of normal (n = 1 line) and TR-KO airway organoids (n = 1 line). Scale bar, 100 mm. Three independent organoids were evaluated with similar results.
