Extended Data Fig. 1: Combined CHD1^Del and SPOP^Mut Promote Prostate Tumorigenesis.

a, Co-occurrence of CHD1^Del and SPOP^Mut in prostate adenocarcinoma (Log2 Odds Ratio > 3; p < 0.001). Oncoprint was generated from cbioportal. b, Quantification of MRI of prostate tumors from 10-month-old male PC (n = 8 mice), PSp (n = 8 mice), and PCSp (n = 19 mice). c, Histopathology analysis of prostate tumors from PC, PSp, and PCSp male GEMM mice. AP: anterior prostate lobes; VP: ventral prostate lobes; DLP: dorsal and lateral prostate lobes. d, Histopathology analysis revealed the total area of carcinoma region in prostate tumors from male PC (n = 8 mice), PSp (n = 8 mice), and PCSp (n = 6 mice). e, IHC staining (Ser473-phosphorylated AKT; CHD1; Flag; AR and Ki67) of prostate tumors from PC, PSp, and PCSp male GEMM mice at 6 months of age. Representative images are from AP lobes. Scale bar = 50 μm. Data (b) are presented as the mean ± standard deviation. Experiments were performed at least three times with similar results. One-way ANOVA with Tukey’s post hoc tests (b) was performed using GraphPad Prism v.9.2.0.

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