Extended Data Fig. 6: SCRN1 stabilizes GPX4 by promoting S45 phosphorylation.
a, Expression of WT and single lysine site mutated GPX4 in HEK293T cells transfected with SCRN1-Myc or control plasmid. b, The structure of GPX4 CMA-target motif (124NVKFD128) and S45 site. The structure of GPX4 was obtained from Protein Data Bank (pdb_00002gs3). c, Detection of the specificity of p-GPX4S45 antibody by ELISA. d, The mRNA expression of SCRN1 and GPX4 in SS and SR PDOs (n = 4 biological replicates). e, Establishment of stably GPX4 KO HepG2 cells. f, Percentage of cell death of WT and GPX4 KO HepG2 cells treated with sorafenib and erastin (n = 3 biological replicates). g, Percentage of cell death of WT and GPX4 KO HepG2 cells with GPX4 reconstitution and treated with sorafenib (n = 3 biological replicates). Data shown are representative of three independent experiments with similar results (a, e). Data presented as mean ± s.d. of three independent experiments and statistical significance was determined by two-tailed unpaired Student’s t-test (d, f, g).
