Extended Data Fig. 2: SMARCA5 is a specific regulator of PDAC initiation without affecting normal pancreas.
a, Representative IHC staining of SMARCA4 and SMARCA5 in PDAC patient samples. Red asterisks indicate tumor cells, and blue arrowheads indicate stromal cells. Data were obtained from The Human Protein Atlas (https://www.proteinatlas.org). Scale bar, 50μm. b, Quantification of SMARCA4 and SMARCA5 intensities in tumor versus stromal cells. Each dot represents a cell (SMARCA5 quantification: n = 29 for tumor cells, n = 25 for stroma cells; SMARCA4 quantification: n = 25 for tumor cells, n = 28 for stroma cells). c, Representative IF staining for SMARCA4 in mouse normal pancreas. White arrows indicate the nuclei of acinar cells. Scale bar, 5μm. d, Representative IF co-staining for GFP and SMARCA5 in Rosa and Smarca5 knockout samples obtained from tumor formation assays described in Fig. 1d. Scale bars, left 200μm, right 20μm. e, Representative IF staining for SMARCA4 in mouse ADM Day 2 samples. Scale bar, 50μm. f, Representative H&E staining in the presence of sgRNAs targeting Trp53, Cdkn2a and the indicated experimental sgRNAs. Scale bar, 100 μm. g, Representative IF co-staining for GFP and SMARCA5 in sgRNA treated samples obtained from survival assay described in Figs. 1f, g at the time of endpoint (mice sacrificed with tumor burden). Scale bar, 50μm. h, Quantification of SMARCA5 intensity in GFP-positive cells from survival assay. n = 3414 cells for sgRosa; n = 5707 cells for sgSmarca5. i, Representative flow cytometric data displaying the percentage of RFP and BFP in Rosa and Smarca5 knockout samples described Fig. 1i at the indicated time points. j, Representative IF co-staining for GFP, RFP and amylase, corresponding to Fig. 1i. Scale bar, 50μm. k, Quantification of amylase intensity in GFP+&RFP+ cells. Significance was determined by one-way ANOVA. n = 3 biological independent mice. Data are present as mean ± SEM, with each dot representing one mouse.
