Extended Data Fig. 4: Intratumor bacteria reside inside cancer cells.

(a–b) Representative images of TMA cores from (a) GBM (70 cores) and (b) brain metastasis samples (86 cores) co stained for bacterial elongation factor EF-Tu (orange), and either GFAP (a) or cytokeratin (b) (red)DAPI is shown in blue. Upper left panel: low-magnification overview with marked regions shown at higher magnification below. Corresponding no primary-antibody controls are shown on the upper right panel. Scale bars in low-magnification images, 200 μm. Scale bars in high-magnification images, 10 μm. For panels a,b images shown are from n = 1 patient per group.