Extended Data Fig. 10: Prevalence of selected genera across brain regions in GBM and brain metastases.
(a-d) Bar plots showing the prevalence of chosen genera from Fig. 6d in occipital and cerebellum tumors compared to frontal tumors in breast cancer and lung cancer brain metastases. P-values were calculated using two-sided Fisher’s exact test. (e) Box plot showing the Shannon diversity index per sample in GBM tumors compared to all brain metastases in anterior (GBM: n = 94 patients, BrM: n = 51 tumor samples from n = 38 patients) and occipital (GBM: n = 7 patients, BrM: n = 16 tumor samples from n = 12 patients) brain regions. P-value was calculated using a two-tailed Student’s t-test. (f) Scatter plot showing the effects of bacterial pre-conditioned media on the response of two human glioma cell lines (T98G and U251) to TMZ treatment. Each point represents one of 30 bacterial species from genera that were identified to be prevalent in GBM tumors (n = 30 bacterial species). The x- and y-axes represent the rescue score (rScore) as explained in the methods section, where positive values represent PCM-mediated resistance and negative values represent an additive or synergistic effect between the PCM and TMZ. Colored dots represent different bacterial genera. A linear regression line is shown to illustrate the relationship between responses across cell lines. BrM, All brain metastases samples. BCBrM, Breast cancer brain metastases samples. LCBrM, Lung cancer brain metastases samples. for panels a-d: frontal: n = 35 tumor samples from n = 29 patients, occipital: n = 16 tumor samples from n = 12 patients, cerebellum: n = 28 tumor samples from n = 19 patients.
