Extended Data Fig. 8: Key marker genes, GO terms and reference data projection of GD2 CAR T cell clusters.
a, Dot plot showing key marker gene expression (selected from the top 20 DEGs) across the GD2 CAR T cell clusters. Dot size is proportional to the percentage of cells expressing a gene and color intensity to the average scaled gene expression. Grid colors highlight genes that are closely related in function; HLA genes (green), metabolic stress-related genes (red) and ISGs (blue). b–f, Selected significant GO terms associated with the DEGs of the TUND (b), TIL-2 (c), TMI (d), TPR (e) and TMS (f) GD2 CAR T cell clusters. g, UMAP visualization of the CD8+ TIL clusters from the pan-cancer atlas46 used as a reference dataset. Annotated clusters are highlighted because of their overlap with, or use in defining the GD2 CAR T cell clusters. h–j, Marker gene signatures (DEG analysis adjusted p-value < 0.00001) of the TUND (h), TIL-2 (i) and TISG (j) GD2 CAR T cell clusters projected onto the CD8+ TIL dataset from g. For panels a-j, GD2 CAR T cells retrieved from n = 4 treated DMGOs.
