Extended Data Fig. 8: Flow cytometry analysis of PC13 CAR T cell phenotype.
Phenotypic characterization of the PC13 CAR T cell product was performed using flow cytometry. Panels A–F show representative flow cytometry plots. a, CAR expression analysis by flow cytometry. b, CD4+ and CD8+ subset distribution. c, Memory subset distribution (stem cell memory [scm], central memory [cm], effector [ef], effector memory [em]). Exhaustion marker profiling, including LAG-3 (d), PD-1 (e), and TIM-3(f). Panels G–I present summary statistics. g, Percentage of CAR+ cells within total CD3⁺ T cells, CD4+, and CD8+subsets. h, Quantification of memory subsets, including relative frequencies of Tscm, Tcm, Tef, Tem. i, Percentage of LAG3+, PD-1+, TIM-3+ cells. Exhaustion panel n = 37 due to insufficient samples in 6 patients. In each boxplot, the central line represents the median, the box edges represent the interquartile range (IQR, 25th–75th percentile), and the whiskers extend to 1.5 × IQR. Each dot represents an individual patient’s PC13 CAR T cell product.
