Fig. 1: Overview of material design, fabrication, and in vivo application of AATIPA–gelatin hydrogels.
From: Non-destructive in vivo tracking of gelatin hydrogels for advancing tissue engineering
A Schematic representation of crosslinking of gelatin derivatives with AATIPA as co-monomer; (B, left) photo, (B, middle) USG fantom, and (B, right) CT fantom of an example of a fabricated 3D-porous scaffold; C depiction of the in vivo implantation/injection of the crosslinked materials and representative CT image of G100I on day 1. The implanted scaffolds were prepared using the negative PLA cylinder-shaped scaffolds with a meander-like pattern consisting of 10 layers, a total height of 3.7 mm, and a diameter of 8.4 mm, and varying pore sizes (approx. 800–100 µm, Table S2). From the resulting 3D-porous scaffolds, cylindrical shapes were punched out with diameters of 0.6 cm and a height of 0.4 cm. The injectables were prepared by freeze-drying crosslinked hydrogel films, followed by grinding them into a fine powder and sieving the powder through a 120 µm industrial sieve. The powder was reswollen in sterile physiological saline and 100–200 µL of the resulting particle solution was injected.
