Fig. 4: Biocompatibility assessment of cell-laden SSOT constructs. | Communications Materials

Fig. 4: Biocompatibility assessment of cell-laden SSOT constructs.

From: A smart self-oxygenating system for localized and sustained oxygen delivery in bioengineered tissue constructs

Fig. 4

In vitro Functional evaluation of cell growth within the hydrogels with and without the SSOT technology at Day 14 via F-Actin/DAPI fluorescence assay and Live/Dead assay considering MSCs, HUVECs, and HDFs under normoxic, hypoxic (5% O2), and anoxic (1% O2) conditions for A 0.1 cm and B 0.5 cm constructs. Scale bar = 200 μm. Quantification of metabolic activity, relative fluorescence units (RFU) via PrestoBlue assay, ATP content, and DAPI-stained cell nuclei for C 0.1 cm constructs and D 0.5 cm constructs. E The total amounts of secreted VEGF and DNA were quantified and used to determine the VEGF/DNA ratio of hMSC-encapsulated SSOT constructs cultured under normoxic, hypoxic, and anoxic conditions. F In vivo biocompatibility analysis with and without SSOT technology via Hematoxylin and Eosin (H&E), Masson’s Trichrome (TRI), Human Nuclear Antigen (HNA)/C-reactive Protein (CRP), CD31/DAPI, and TUNEL/DAPI assays. Scale bar = 200 μm. Data represent means ± SEM (n = 4) with statistical significance indicated by P values from two-way ANOVA and Tukey’s multiple comparisons test (ns–no significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).

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