Fig. 5: Temporal patterns of predicted microbial functions, metabolomes, and DOM compositions in groundwater.
From: Groundwater microbiomes balance resilience and vulnerability to hydroclimatic extremes
a Temporal similarity of functional profiles predicted by PICRUSt2 using 16S rRNA gene-sequences; b Temporal similarity in groundwater metabolomes, shown alongside a subset of concurrently sampled groundwater microbiomes; c Temporal similarity of groundwater DOM compositions alongside a subset of concurrently sampled groundwater microbiomes. Bray-Curtis similarity (%) was used to assess temporal similarity in all panels. Points represent raw Bray-Curtis similarity values plotted against pairwise sampling time intervals. Regression lines and smoothing curves are added to visualize both linear turnover and potential sinusoidal trends. Mantel tests were conducted to assess correlations a between changes in predicted pathway and time; b between changes in microbiomes and metabolomes; c between changes in microbiomes and DOM. Sample sizes per well for mantel tests (a/b/c) are as follows: H14, n = 89/32/20; H32, n = 125/58/34; H41, n = 117/57/34; H43, n = 118/57/34; H51, n = 119/61/33; H52, n = 124/63/36; H53, n = 123/59/35.
