Extended Data Fig. 5: CN1 and CN2 show inhibitory activity against the pests’ Naam enzymes.
From: Pharmacophore-oriented 3D molecular generation toward efficient feature-customized drug discovery
a, b IC50 curves are obtained by incubating compounds CN1 and CN2 with the pests’ Myzus persicae Naam (15 nM) and Bemisia tabaci Naam (30 nM) enzymes, respectively, for durations ranging from 0 to 120 minutes, revealing that both compounds are time-dependent inhibitors of these Naam enzymes; data are presented as mean values ±SEM from n = 3 biological replicates, with error bars representing the SEM. c, d The melting curves (first-derivative of dissociation) of Myzus persicae Naam (10 μM) and Bemisia tabaci Naam (10 μM) in presence or absence of CN1 (50 μM) or CN2 (50 μM), reveal that these two compounds bind to and stabilize these enzymes; data are presented as mean values ±SEM from n = 3, 2, 3, 3 for c and n = 3, 2, 3, 1 for d biological replicates, with error bars representing the SEM.
