Fig. 3: Regulation of innate immune response genes.

a, b Relative normalized expression of the RIG-I-like receptors family members DHX58 (a), DDX58 and IFIH1 (b) in vaccinated and unvaccinated patients. A two-tailed unpaired t-test with Welch’s correction was used to evaluate the statistical significance between the two groups. *p < 0.05 c, d DHX58 (c), DDX58 and IFIH1 (d) mRNA levels from primary bronchial cells treated with IFNs were measured by RNA-seq12. Results are shown as the means ± s.e.m. of independent biological replicates (n = 3). ****p < 0.00001 e ChIP-seq for STAT transcription factors, the histone marks H3K27ac and H3K4me3, and Pol II was conducted in primary bronchial cells in the absence and presence of IFNβ. f mRNA levels of IFIT gene family members in vaccinated and unvaccinated patients. A two-tailed unpaired t-test with Welch’s correction was used to evaluate the statistical significance between the two groups.