Fig. 1: Schematic showing EV isolation workflows using either Verita™ or ultracentrifugation methods.
From: Early-stage multi-cancer detection using an extracellular vesicle protein-based blood test
a Workflow using the Verita™ Isolation platform. As plasma samples are flowed onto the energized AC Electrokinetics (ACE) microelectrode array, EVs are collected onto the electrodes. Unbound materials are removed with a buffer wash, the electric field turned off, and EVs are eluted into the buffer. b Workflow for differential ultracentrifugation. Plasma samples are diluted, and large debris pelleted by a low-speed centrifugation step. Supernatants are removed and subjected to two additional cycles of low-speed centrifugation. EVs in the cleared supernatants are then ultracentrifuged two times, and lastly the pellet is resuspended in buffer.
