Fig. 5: Performance of Au-PEG6-VHH-72-C13 and Au-VHH-72-C13 electrodes on cultured SARS-CoV-2 EU variant.
From: SARS-CoV-2 detection using a nanobody-functionalized voltammetric device
A Dose-dependent response curves of 10-fold dilutions (D1-D9) of SARS-CoV-2, clade 20 A.EU2 (EU variant) on VHH-72-13C modified electrodes and VHH-72 for comparison. B Correlation of qRT-PCR Ct values and viral RNA copies mL−1. C Correlation of viral RNA copies mL−1 with plaque forming units of SARS-CoV-2 as a measure of infectivity. For this, Vero E6 cells were infected with 10-fold dilutions of a SARS-CoV-2 isolate clade 20 A.EU2 (EU variant). Calculation of estimated virus concentration was carried out by the Spearman and Karber method20,21 and expressed as TCID50/mL (50% tissue culture infectious dose). TCID50/mL values were transformed to PFU mL−1 by using the formula PFU mL−1 = TCID50/mL × 0.7 (https://www.lgcstandards-atcc.org/support/faqs/48802/Converting%20TCID50%20to%20plaque%20forming%20units%20PFU-124.aspx). RNA extraction and qRT-PCR (target IP4) were performed in duplicate for each dilution. D Dose-dependent response curves of viral copies of SARS-CoV-2, clade 20 A.EU2 (EU variant) on VHH-72-13C modified electrodes. The results are expressed as the mean ± SEM of at least 3 independent electrodes for each group.
