Fig. 2: Competitive equilibrium-based dissociation of ZOL by 800CW-pRIS in vitro. | Communications Medicine

Fig. 2: Competitive equilibrium-based dissociation of ZOL by 800CW-pRIS in vitro.

From: Fluorescent risedronate analogue 800CW-pRIS improves tooth extraction-associated abnormal wound healing in zoledronate-treated mice

Fig. 2

a Experimental plan to examine the competitive equilibrium-based dissociation of N-BP (5-FAM-ZOL) by 800CW-pRIS. 5-FAM-ZOL (10 µM) was pre-adsorbed to synthetic apatite wells, which were challenged by 800CW-pRIS (10 µM in MilliQ filtered pure water: MQW) one or two times. For control application (Cont), the vehicle (MQW) was applied. b Fluorescent FAM and 800CW signals of the synthetic apatite wells. FAM signal was decreased after 800CW-pRIS applications, while 800CW signal was increased. c FAM signal of the recovered solutions after each treatment. FAM signal was increased after 800CW-pRIS application, suggesting the removal of 5-FAM-ZOL from the synthetic apatite well. The in vitro experiment was performed in triplicated wells per group (b, c). The graphs present the mean and standard deviation. Student’s t test was used to compare the 5-FAM-ZOL alone group and all other groups (b) or the 5-FAM-ZOL along group and all other groups within the indicated time points (c) (n = 3 for each group set). *p < 0.05; **p < 0.01. Source data: Supplementary Data 1, Fig. 2b, c.

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