Fig. 4: Detection of drug concentration-dependent toxicity and liver injury.
From: Performance assessment and economic analysis of a human Liver-Chip for predictive toxicology
Effect of Cloazpine (closed circles) or olanzapine (open circles) on albumin production (a), ALT release (b), and morphology score (c); Effect of troglitazone (closed circles) or pioglitazone (open circles) on albumin production (d), ALT release (e), and morphology score (f); Effect of trovafloxacin (closed circles) or levofloxacin (open circles) on albumin production (g), ALT release (h) and morphology score (i); Immunofluorescence microscopic images showing concentration-dependent increases in caspase 3/7 staining (green;) indicative of apoptosis after treatment with trovafloxacin at 0,1, 10, and 100 (j) times the unbound human Cmax for 7 days; concentration-dependent decrease in TMRM staining (yellow) indicative of mitotoxicity in response to treatment with sitaxsentan at 0,1,10, and 100 (k) times the unbound human Cmax for 7 days. Scale bar represents 50 µm.
