Fig. 4: Formulation and environmental humidity influence residual moisture content and long-term stability of AAV within the film matrix.
From: Thermostability and in vivo performance of AAV9 in a film matrix
a Residual moisture in high (F1S) and low (F2S) viscosity films after drying. Data represent the average ± the standard error of the mean for three replicates for each formulation. **p < 0.01, two-tailed Student’s t-test. b Sixty percent relative humidity (yellow bars) maintains AAV stability within the film matrix. Films containing 1 × 1012 virus genomes were prepared in batches of the high-viscosity formulation (F1S) and stored at 25 °C in different environments with respect to humidity. Films (3 per timepoint and storage condition) were rehydrated with warm culture media and infectious titer was determined by limiting dilution. *p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA with Tukey’s post hoc multiple comparison tests.
