Fig. 4: Association of neutralizing antibody response with infecting DENV serotype.

The four DENV serotypes are represented by the following colours in all the figure panels depicting FRNT data: DENV-1:grey, DENV-2:pink, DENV-3:green, DENV-4:dark purple. a Neutralizing antibody titers against any one of the four serotypes of DENV, as estimated by FRNT assay in a subset of samples (n = 31). b Neutralizing antibody titers against two or more serotypes of DENV as estimated by FRNT assay in a subset of samples (n = 149). Mean rank of each column was compared with mean rank of every other column using non-parametric Kruskal-Wallis test. Correction for multiple comparisons was applied by Dunn’s multiple comparison test. *P = 0.0213; ***P = 0.0002; ****P < 0.0001. c Distribution of infecting dengue serotypes from the samples showing multitypic neutralizing antibody response represented by the colours: blue – DENV-1, red-DENV-2, green – DENV-3, purple – DENV4, orange - indeterminate serotype). d–h FRNT₅₀ values of samples classified based on the infecting DENV serotype or serotype indeterminate samples. Mean rank of each column was compared with mean rank of every other column using non-parametric Kruskal-Wallis test. Correction for multiple comparisons was applied by Dunn’s multiple comparison test.***P = 0.0009 in (d). *P = 0.0483 in (e). *P = 0.0443 for DENV-1 vs DENV-4 and P = 0.0101 for DENV2- vs DENV-3 in (f). P = 0.0012 in (g). P = 0.0030 for DENV-2 vs DENV-3 and P = 0.0002 for DENV-2 vs DENV-4 in (h). LOQ is limit of quantitation of the assay. i, j FRNT₅₀ values for all the four dengue virus serotypes in samples classified as primary or secondary dengue infection respectively. Mean rank of each column was compared with mean rank of every other column using non-parametric Kruskal-Wallis test. Correction for multiple comparisons was applied by Dunn’s multiple comparison test. ns: not significant. **P = 0.0020; ***P = 0.0006; ****P < 0.0001. “n” in each of the panels indicates biologically independent samples. Error bars represent Min and Max with range. P-values are indicated only for observations where the differences are statistically significant.