Fig. 6: IHC of MHC-II in host and graft RPE cells after transplantation.
From: Graft survival of major histocompatibility complex deficient stem cell-derived retinal cells
a Retinal section of a normal retina (the left eye of S2-4 monkey without RPE transplantation) was stained with MHC-II (red) and iba1 (green). b Retinal section of the right eye of S2-4 monkey that showed rejection after transplantation with wild-type RPE (1121A1 iPSC-derived) was stained after 6 months of transplantation with MHC-II (red) and iba1 (green). c Retinal section of K-177 monkey that showed rejection after transplantation of PKH (red)-prelabeled wild-type RPE (46a iPSC-derived) was stained after 6 months of transplantation with MHC-II (green). d Retinal section of HM-23 monkey that did not show rejection after transplantation of PKH (red)-prelabeled MHC-II knockout RPE (1121A1 iPSC-derived) was stained after 6 months of transplantation with MHC-II (green). DAPI (blue) was used for counterstaining. PKH (red) pre-labeling of the grafts was faint after 6 months of transplantation (c, d). MHC-II was detected in wild-type-transplanted (b, c) but not in normal (a) or MHC-II knockout-transplanted (d) retinas. Upper panels are merged images with bright filed that show pigmented RPE layer. White arrows: host RPE layer, yellow arrows: graft RPE, dashed line circles: double-stained cells, yellow arrowheads: blood endothelial cells. INL inner nuclear layer, ONL outer nuclear layer, RPE retinal pigment epithelium. Scale bars: 20 μm.
