Fig. 2: Immunohistochemical assessment of complexes I and IV in PD single muscle fibers. | Communications Medicine

Fig. 2: Immunohistochemical assessment of complexes I and IV in PD single muscle fibers.

From: Mitochondrial complex I deficiency occurs in skeletal muscle of a subgroup of individuals with Parkinson’s disease

Fig. 2

Complex I (NDUFB10); (a, b) and complex IV (MTCO1); (c, d) fluorescence intensity normalized to mitochondrial mass (VDAC1) in single muscle fibers (n = 75–100 per individual) from 71 individuals with PD (red) and 21 neurologically healthy controls (blue). VDAC1 fluorescence intensity measurements from the same fibers are shown (e, f). Values are log transformed. For the purpose of visualization, the data have been adjusted for the effect of staining batch by regressing out this variable (see “Methods” section). Boxplots (a, c, e) show individual-level distributions of single fiber measurement where each box represents one individual. Boxes: median and interquartile range (IQR); whiskers: 1.5 x IQR from the lower and upper quartiles. Individuals are sorted by median values from left to right. Dashed lines show the group-level medians of the PD and control groups. The histograms (b, d, f) represent group-level distributions of single fiber measurement in the PD and control groups. Supplementary Fig. 8 shows complex I and complex IV data without adjusting for staining batch. A representative example of quadruple immunohistochemistry in a skeletal muscle biopsy from an individual with PD shows fluorescence signal for NDUFB10 (g), MTCO1 (h), the mitochondrial mass marker VDAC1 (i) and laminin (j). Yellow borders represent regions of interest (ROIs) used for measuring fluorescence signal in 100 single muscle fibers. Zoomed-in regions are indicated by white squares. Scale bar: 200 µm.

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