Table 1 Correction factors for sample preparation

From: Impact of clinical preparation steps and use of sex-specific reference for accurate antibiotic monitoring in body fluids

Preparation method

Factor/%

RMSEP/µM

RMSEP*/µM

froz

8.42 ± 0.04

57.8

10.4

filt

2.36 ± 0.04

14.7

14.1

cent

2.80 ± 0.10

33.5

29.8

filt + cent

5.05 ± 0.06

45.4

12.1

  1. Correction factor for the effect of freezing (froz), centrifugation (cent), filtration (filt), and their combination (filt + cent) using PLS regression model. Since the factor evaluation is based on the RMSEP, this value is also listed before and after (*) minimization. Freezing has the highest effect on concentration changes, centrifugation and filtration are lower, but when applied sequentially, they are additive. The error of the respective factor results from the 10-fold cross-validation of the predicted data. In each case, 80% of the data was used and the factor was calculated according to Eq. (1). The specified value corresponds to the median and the respective median absolute deviation.
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