Fig. 2: Structural and functional characterizations of hCiCMs. | Communications Medicine

Fig. 2: Structural and functional characterizations of hCiCMs.

From: Reprogramming of human urine cells into cardiomyocytes via a small molecule cocktail in xeno-free conditions

Fig. 2: Structural and functional characterizations of hCiCMs.

a Immunofluorescence analyses of CM markers, n = 3. Scale bars, 50 µm. b Transmission electron microscopy images of hCiCMs derived from hUCs at day 60, n = 3. MF myofibrils, Z Z-bands, Mit mitochondria. c RT-qPCR analysis of mRNA levels of genes related to pluripotency markers, cardiac structure, ion channels, and mitochondrial functions of hCiCMs derived from hUCs on day 60, n = 3. d Representative action potentials (AP) of hCiCMs derived from hUCs on day 60, n = 15. Em, membrane potential in millivolts. e AP parameters of hCiCMs including AP frenquency, AP amplitude (APA), minimum diastolic potential (MDP), AP durations (APDs) at the level of 30% (APD30), AP durations (APDs) at the level of 50% (APD50) and 90% repolarization (APD90), n = 15. Data are means ± SD. f Representative calcium flux in human cardiac-induced cardiomyocytes (hCiCMs) derived from human umbilical cord stem cells (hUCs) at day 60 of induction, n = 6. g Measurement of the maximum calcium transient amplitude, maximum upstroke velocities, and maximum decay velocities of calcium transients in hCiCMs derived from hUCs on day 60 of induction (n = 6). Data are presented as means ± SD.

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