Fig. 2: EV surface antigen profiling after immuno-capturing in rejecting recipients. | Communications Medicine

Fig. 2: EV surface antigen profiling after immuno-capturing in rejecting recipients.

From: Machine learning-assisted assessment of extracellular vesicles can monitor cellular rejection after heart transplant

Fig. 2

Standardized profiling of surface antigens after EV isolation by immuno-capturing. Normalized median fluorescence intensity (nMFI; expressed as an arbitrary unit, a.u.) is reported for tetraspanins (CD9, CD63, and CD81) and for a predefined panel of 37 markers commonly expressed on EV membrane (285 samples included in the analysis). a Protocol for EV profiling after immuno-capturing. b Heat map showing EV surface antigen expression in patients stratified for rejection grade (from 0 to 3A; blue, low fluorescence; red, high fluorescence levels). c, d Median expression levels of EV surface antigens differentially expressed in rejecting patients, after patient stratification for time point of evaluation and rejection grade (n = 285): after surgery (first sampling after heart transplant); grade 0 (non-rejecting patients); ACR grade 1A/B; pre 1A/B (time point of evaluation before a diagnosis of ACR grade 1A/B); ACR grade 2–3A; pre 2–3A (time point of evaluation before a diagnosis of ACR grade 2–3A). Source data and statistics are reported in Supplementary Data S2 and S3. Part of (a) was produced using the Servier Medical Art public domain (https://smart.servier.com).

Back to article page