Fig. 3: E-cigarettes elicit differential gene expression dependent on fetal sex. | Communications Medicine

Fig. 3: E-cigarettes elicit differential gene expression dependent on fetal sex.

From: Flavored e-cigarettes modulate embryo development, fetal growth, and potentiate early fetal demise without nicotine

Fig. 3: E-cigarettes elicit differential gene expression dependent on fetal sex.

Gene expression was performed on e-cigarette-exposed, sexed placental tissue at day 12.5 (a–f). Two-way ANOVA was performed with Tukey’s multiple comparisons test. Grubbs' Outlier test was used when appropriate. SHAM depicted as white circles on black bars; e-cigarette vapors without nicotine (VAPE) depicted as grey circles on grey bars; e-cigarette vapors with nicotine (VAPE NIC) depicted as pink circles on pink bars (a–f). Error bars indicate mean ± SEM. p = adjusted p-value. Red font p-values indicate significance with a p-value\(\le\)0.05. N numbers: Hif1a (compiled: N = 8 SHAM, N = 8 VAPE, N = 7 VAPE NIC; male, female: N = 4), Ptgs2 (compiled: N = 8; male, female: N = 4), Gpx2 (compiled: N = 8; male: N = 4; female: N = 4 SHAM, N = 3 VAPE, N = 4 VAPE NIC), Gpx3 (compiled: N = 8 SHAM, N = 7 VAPE, N = 7 VAPE NIC; male: N = 4 SHAM, N = 3 VAPE, N = 3 VAPE NIC; female: N = 4), Txnrd1 (compiled: N = 8; male, female: N = 4), Mapk1 (compiled: N = 8 SHAM, N = 8 VAPE, N = 7 VAPE NIC; male, female: N = 4).

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