Table 3 Two-way ANOVA analysis of individual bisphenol A dose (BPA0.5 and BPA50) on the plasma metabolome

BPA0.5	2-way ANOVA			Direction		Post-hoc Tukey
NMR name	BPA	sex*BPA	p-value	Male	Female	p-value	Change
Main		ABA1	0.0273	Up		0.0347	mBPA0.5 vs all
ApoB		L6PN	0.00629			0.146
Main V/L/H		HDPL	0.0122			0.176
Sub VLDL		V5CH	0.0482	Up	Down	0.00525	mBPA0.5 vs fBPA0.5
		V5PL	0.0135	Up	Down	0.0233	mBPA0.5 vs fBPA0.5
Sub LDL		L6TG	0.0427	Up	Down	0.0003	mBPA0.5 vs fBPA0.5
		L6CH	0.00124	Up		0.0307	mCont vs mBPA0.5
		L6FC	0.00248			0.0516
		L6PL	0.00219	Up		0.0413	mCont vs mBPA0.5
		L6AB	0.00630			0.147
Sub HDL		H3CH	0.0252			0.322
		H3PL	0.00056	Down		0.0269	mCont vs mBPA0.5
		H3A1	0.0240			0.124
Small Metab	Ile		0.00632	Up		0.0031	mBPA0.5 vs fCont
		Leu	0.0449	Up	Down	0.0464	fCont vs mCont
		Formate	0.0403	Down	Up	0.0325	mCont vs fCont

BPA50	2-way ANOVA			Direction		Post-hoc Tukey
NMR name	BPA	sex*BPA	p-value	Male	Female	p-value	Change
ApoB		L2PN	0.0123			0.173
Sub LDL		L2CH	0.0138	Up	Down	0.00016	fCont vs mCont
		L2FC	0.00942	Up	Down	0.00016	fCont vs mCont
		L2PL	0.00248	Up	Down	0.00016	fCont vs mCont
		L2AB	0.0123			0.172
Small Metab		Gly	0.0389			0.197
	Phe		0.0200	Up		0.0484	mBPA0.5 vs fCont

The table shows the lipoprotein identity and significant changes caused by individual developmental BPA-dose exposure. Post-hoc analysis indicates the Direction (up or down) of change, and Change indicates the statistically significant comparison
NMR (see legend of Supplementary Fig. 3): ABA1 (Apo-B1/Apo-A1), L6PN (high-density LDL, particle number), HDPL (HDL, phospholipids), V5CH/PL (high-density VLDL, cholesterol/phospholipid), L6TG/CH/FC/PL/AB (high-density LDL, triacylglycerol/cholesterol/free cholesterol/phospholipid/Apo-B1), H3CH/PL/A1 (HDL, cholesterol/phospholipid/Apo-A1), L2PN/CH/FC/PL/AB (low-density LDL, particle number/cholesterol/free cholesterol/phospholipid/Apo-B1), Ile (isoleucine), Leu (leucine), Gly (glycine) and Phe (phenylalanine). f (female), m (male), Cont (controls). Exposure groups: BPA0.5 (0.5 μg BPA/kg BW/day) and BPA50 (50 μg BPA/kg BW/day). Number of animals/group: control females n = 11, BPA0.5 exposed females n = 8, BPA50 exposed females n = 7, control males n = 12, BPA0.5 exposed males n = 8, BPA50 exposed males n = 8. Two-way ANOVA, followed by the post-hoc Tukey test.

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