Fig. 1: Overexpression of CYP27A1 and downregulation of CYP7B1 in LUAD cells of patients lead to 27-hydroxy-cholesterol accumulation and activated pathways that drive progression and metastasis through NFkB p65, AKT, SNAIL, and Vimentin. | Communications Medicine

Fig. 1: Overexpression of CYP27A1 and downregulation of CYP7B1 in LUAD cells of patients lead to 27-hydroxy-cholesterol accumulation and activated pathways that drive progression and metastasis through NFkB p65, AKT, SNAIL, and Vimentin.

From: Dietary ω−3 polyunsaturated fatty acids (PUFAs) reduce cholesterol-driven non-small cell lung cancer (NSCLC) progression in mouse models of disease

Fig. 1: Overexpression of CYP27A1 and downregulation of CYP7B1 in LUAD cells of patients lead to 27-hydroxy-cholesterol accumulation and activated pathways that drive progression and metastasis through NFkB p65, AKT, SNAIL, and Vimentin.The alternative text for this image may have been generated using AI.

RNA was isolated from the lung tumoral (TU) and control (CTR) region of patients with LUAD. qPCR was performed and mRNA expression level was related to the housekeeping gene RPL30. A CYP27A1 mRNA expression in control (n = 25) and tumoral (n = 27) region. P (CTR vs. TU) = 0.0163. B CYP7B1 RNA expression in control (n = 33) and tumoral (n = 28) region. P (CTR vs. TU) = 0.0006. C PPIB RNA expression in control (n = 9) and tumoral (n = 7) region. P (CTR vs. TU) = 0.0312. D NFkB p65 RNA expression in control (n = 26) and tumoral (n = 28) region. P (CTR vs. TU) = < 0.0001. E AKT RNA expression in control (n = 24) and tumoral (n = 25) region. P (CTR vs. TU) = 0.0277. F SNAIL RNA expression in control (n = 8) and tumoral (n = 7) region. P (CTR vs. TU) = 0.0312. G Vimentin RNA expression in control (n = 16) and tumoral (n = 15) region. P (CTR vs. TU) = 0.0413. H Correlation of relative ACAT1/RPL30 mRNA expression level measured in the control/tumoral region correlated with relative CYP27A1/RPL30 mRNA expression level measured in control/tumoral region of patients with LUAD; CTR (n  =  24, p  =  0.0334, R2  =  0.1979), TU (n  =  27, p  =  0.0052, R2  =  0.3044). I Correlation of relative NFkB p65/RPL30 mRNA expression level measured in the control/tumoral region correlated with relative CYP27A1/RPL30 mRNA expression level measured in control/tumoral region of patients with LUAD; CTR (n  = 25, p  =  0.0028, R2  =  0.3269), TU (n  =  26, p  =  0.0005, R2  =  0.4054). J Correlation of relative AKT/RPL30 mRNA expression level measured in the control/tumoral region correlated with relative NFkB p65/RPL30 mRNA expression level measured in control/tumoral region of patients with LUAD; CTR (n  = 22, p  =   < 0.0001, R2  =  0.6141), TU (n  =  24, p  =   < 0.0001, R2  =  0.7582). K Correlation of relative Vimentin/RPL30 mRNA expression level measured in the control/tumoral region correlated with relative NFkB p65/RPL30 mRNA expression level measured in control/tumoral region of patients with LUAD; CTR (n  = 16, p  =  0.0016, R2  =  0.5212), TU (n  =  15, p  =  0.0006, R2  =  0.6073). L Experimental setup of wound healing assay. [Created in BioRender. Harre, P. (2025) https://BioRender.com/j76f918]. M % of remigrated area 48 h after scratching confluent cell surface. Extent of remigration was defined by the ratio of the difference between the remaining wound areas compared with the original wound area. Conditions used were control (CTR; n = 8) and α-linolenic acid (α-LA; n = 8) stimulated. P (CTR vs. α-LA) = 0.0047. Correlations (H–K) are shown by using simple linear regression. The two-tailed Pearson correlation analysis was performed to get the r and p value for (H–K). Wilcoxon test was performed for (A, C–G). Paired t-test was performed for (B). Mann-Whitney test was used for (M). All statistical tests were two-sided. No adjustments for multiple comparisons were applied. Data are shown as mean values  ±  s.e.m.; *P  <  0.05; **P  <  0.01 ***P  <  0.001; ****P  <  0.0001.

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