Fig. 5: Validation of Hub Genes. | Communications Medicine

Fig. 5: Validation of Hub Genes.

From: Imaging and RNA sequencing-based radiogenomic analysis of occult lymph node metastasis and survival in lung adenocarcinoma staged non-metastatic

Fig. 5

A The expression of hub-lncRNAs in LUAD tissue samples of OLMN, LUAD tissue samples of OLMP and corresponding noncancerous tissue samples (nomal) were detected using RT-qPCR. B The levels of MIR600HG expression in 5 human LUAD cell lines and 1 human bronchial epithelial cell line were measured by RT-qPCR. C, D Knockdown efficiency of MIR600HG in C A549 and D H1299 cells determined by RT-qPCR. E, F Validation of pcDNA3.1-MIR600HG over-expression vector in E A549 and F H1299 cells as determined by RT-qPCR. LUAD lung adenocarcinoma, OLMN occult lymph node metastasis negative, OLMP occult lymph node metastasis positive, RT-qPCR reverse transcription and quantitative real-time polymerase chain reaction. Horizontal lines (A–F) represent mean, and error bars represent standard deviation (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, nsP > 0.05, unpaired two-sided t test). Each experiment was repeated three times independently (n = 3 biologically independent experiments).

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