Fig. 1: Autoantibody repertoire analysis and validation in paraneoplastic pemphigus.

Characterization of the autoantibody repertoire in paraneoplastic pemphigus was conducted using a two-step approach. In the discovery phase, a cohort of 14 cases of paraneoplastic pemphigus, eight cases of Ap200P, and 18 healthy controls were screened with a microarray featuring more than 9000 full-length human recombinant proteins. In the second phase, the newly identified putative autoantigens were validated using a bead-based array in an extended cohort of paraneoplastic pemphigus patients, in patients with non-paraneoplastic blistering diseases, cancer, and in healthy controls.