Extended Data Fig. 4: Confocal microscopy analysis of the localization of PAS variants expressed in P. pastoris. | Nature Synthesis

Extended Data Fig. 4: Confocal microscopy analysis of the localization of PAS variants expressed in P. pastoris.

From: Biosynthesis of catharanthine in engineered Pichia pastoris

Extended Data Fig. 4

Confocal microscopy analysis of the localization of PAS variants expressed in P. pastoris. The vacuole was targeted by eGFP (PEP4sp-eGFP), while PAS variants were fused with mCherry and their localization was analyzed by comparing the green (500/540 nm) and red (570/670 nm) fluorescence. Yeast cells were cultured in YPM medium until mid-log phase and subject to confocal microscopy analysis (OLYMPUS IX83-FV3000). The results represent the mean ± s.d. of biological triplicates (n = 3).

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