Extended Data Fig. 8: Mass spectrometric analysis of production of acylated ACP.
From: Discovery and biosynthetic pathway analysis of cyclopentane–β-lactone globilactone A
a holo-ACP (GloB). b ACP-tethered linear substrate (8-S-ACP) from pantetheine-activated (8c) via in vitro CoA biosynthesis and Sfp-mediated loading. c holo-GloC-GloD, holo-ACP-C-FkbH (GloB), 1,3-BPG, NADPH and 8-S-ACP. Additional peaks marked by asterisks represent adventitious gluconylation (+178 Da) of N-terminal His6-tag during expression of the recombinant protein in E. coli.
