Fig. 1: Overview of previous studies and this work.
From: An engineered aldolase enables the biocatalytic synthesis of 2′-functionalized nucleoside analogues
a, Examples of 2′-modified nucleoside and nucleotide analogues. b, Examples of common 2′-modifications made to therapeutic oligonucleotides. c, Nucleoside salvage pathway enzymes involved in the breakdown and recycling of nucleosides. d, Previously published biocatalytic synthesis of islatravir8 using engineered variants of salvage pathway enzymes. e, This work: proposed synthesis of 2′-functionalized nucleosides using non-natural aldol donors resulting in modifications (R group) at the 2′-position. NB, nucleobase.
