Extended Data Fig. 5: Characterization of the 3xHA-REV-ERBα cistrome in the heart.
a, Venn diagram showing the overlap between the downregulated differentially expressed genes (DEGs) in CM-RevDKO hearts and up/downregulated genes in cardiomyocyte-specific glucocorticoid receptor (GR) KO hearts32. b, Pearson correlation plots comparing 3xHA ChIP-seq replicate samples. c, Pie chart of annotated cardiac 3xHA-REV-ERBα ChIP-seq peaks. d, Results of motif search at 3xHA-REV-ERBα ChIP-seq peaks and enhancers that displayed increased H3K27ac Cut&Run signal (FC > 2) in CM-RevDKO vs control cardiomyocytes as reported by HOMER. e, Venn diagram showing overlap between DEGs in CM-RevDKO vs control hearts and annotated peaks from the cardiac 3xHA-REV-ERBα cistrome (at ZT10). f, ChIP-seq, Cut&Run and RNA-seq read distribution for REV-ERBα and H3K27ac near derepressed REV-ERBα canonical target genes Bmal1 and g, Cry1 and h, output genes p21 and Fbn2. i, Immunoblot and quantification for FLAG and REV-ERBα from DsRed (control) vs FLAG-Rev-erbα overexpressing plasmid transfected C2C12 cells (n = 2 independently transfected wells/condition). Significance of overlap in e is calculated via a hypergeometric test without multiple testing correction.
